Genetically Encoded Protein Phosphorylation in Mammalian Cells.

ABSTRACT: Protein phosphorylation regulates diverse processes in eukaryotic cells. Strategies for installing site-specific phosphorylation in target proteins in eukaryotic cells, through routes that are orthogonal to enzymatic post-translational modification, would provide a powerful route for defining the consequences of particular phosphorylations. Here we show that the SepRS^v1.0/tRNA^v1.0_CUA pair (created from the Methanococcus maripaludis phosphoseryl-transfer RNA synthetase [MmSepRS]/Methanococcus janaschii [Mj]tRNA_GCA^Cys pair) is orthogonal in mammalian cells. We create a eukaryotic elongation factor 1 alpha (EF-1α) variant, EF-1α-Sep, that enhances phosphoserine incorporation, and combine this with a mutant of eRF1, and manipulations of th

SUBMITTER: Beranek V 

PROVIDER: S-EPMC6162345 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature