Conformational Design and Characterisation of a Truncated Diamine Oxidase from Arthrobacter globiformis.

ABSTRACT: A functional mini protein can be developed by miniaturising its size. The minimisation technique provides an excellent model system for studying native enzymes, especially in creating an alternative novel biocatalyst. Miniaturised proteins may have enhanced stability, a crucial characteristic for large-scale production and industrial applications. In this study, a huge enzyme molecule, known as diamine oxidase (DAO, comprising 700 amino acids), was selected to undergo the process. By retaining the arrangement of the original functional sites of DAO in the fourth domain, a mini DAO can be designed via homology modelling. After several downsizing processes, a final configuration of 220 amino acids displayed high binding affinity towards histamine, a short-chain substrate that was catalysed b

SUBMITTER: Razali NN 

PROVIDER: S-EPMC6163217 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature