Discovery of lahS as a Global Regulator of Environmental Adaptation and Virulence in Aeromonas hydrophila.

ABSTRACT: Aeromonas hydrophila is an important aquatic microorganism that can cause fish hemorrhagic septicemia. In this study, we identified a novel LysR family transcriptional regulator (LahS) in the A. hydrophila Chinese epidemic strain NJ-35 from a library of 947 mutant strains. The deletion of lahS caused bacteria to exhibit significantly decreased hemolytic activity, motility, biofilm formation, protease production, and anti-bacterial competition ability when compared to the wild-type strain. In addition, the determination of the fifty percent lethal dose (LD₅₀) in zebrafish demonstrated that the lahS deletion mutant (ΔlahS) was highly attenuated in virulence, with an approximately 200-fold increase in LD₅₀ observed as compared with that

SUBMITTER: Dong Y 

PROVIDER: S-EPMC6163582 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature