ATP-Induced Increase in Intracellular Calcium Levels and Subsequent Activation of mTOR as Regulators of Skeletal Muscle Hypertrophy.

ABSTRACT: Intracellular signaling pathways, including the mammalian target of rapamycin (mTOR) and the mitogen-activated protein kinase (MAPK) pathway, are activated by exercise, and promote skeletal muscle hypertrophy. However, the mechanisms by which these pathways are activated by physiological stimulation are not fully understood. Here we show that extracellular ATP activates these pathways by increasing intracellular Ca²⁺ levels ([Ca²⁺]_i), and promotes muscle hypertrophy. [Ca²⁺]_i in skeletal muscle was transiently increased after exercise. Treatment with ATP induced the increase in [Ca²⁺]_i through the P2Y₂ receptor/inositol 1,4,5-trisphosphate receptor pathway, and subsequent activation of mTOR in vitro. In addition, t

SUBMITTER: Ito N 

PROVIDER: S-EPMC6163678 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature