Transcriptome Analysis of Novosphingobium pentaromativorans US6-1 Reveals the Rsh Regulon and Potential Molecular Mechanisms of N-acyl-l-homoserine Lactone Accumulation.

ABSTRACT: In most bacteria, a bifunctional Rsh responsible for (p)ppGpp metabolism is the key player in stringent response. To date, no transcriptome-wide study has been conducted to investigate the Rsh regulon, and the molecular mechanism of how Rsh affects the accumulation of N-acyl-l-homoserine lactone (AHL) remains unknown in sphingomonads. In this study, we identified an rsh_US6⁻1 gene by sequence analysis in Novosphingobium pentaromativorans US6-1, a member of the sphingomonads. RNA-seq was used to determine transcription profiles of the wild type and the ppGpp-deficient rsh_US6⁻1 deletion mutant (∆rsh). There were 1540 genes in the Rsh_US6⁻1 regulon, including those involved in common traits of sphingomonads such as exopolysacch

SUBMITTER: Lu H 

PROVIDER: S-EPMC6163740 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature