Does the Pollen Diet Influence the Production and Expression of Antimicrobial Peptides in Individual Honey Bees?

ABSTRACT: We investigated the importance of protein nutrition for honey bee immunity. Different protein diets (monofloral pollen of Helianthus spp., Sinapis spp., Asparagus spp., Castanea spp., a mixture of the four different pollen and the pollen substitute Feedbee^TM) were fed to honey bees in cages ad libitum. After 18 days of feeding, apidaecin 1 isoforms concentration in the thorax were measured using nanoflow liquid chromatography coupled with mass spectrometry. Expression levels of genes, coding for apidaecins and abaecin in the abdomen were determined using quantitative PCR. The results indicate that protein-containing nutrition in adult worker honey bees can trigger certain metabolic responses. Bees without dietary protein showed lower apidaecin

SUBMITTER: Danihlik J 

PROVIDER: S-EPMC6164669 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature