Assessment of the Quorum Sensing Inhibition Activity of a Non-Toxic Chitosan in an N-Acyl Homoserine Lactone (AHL)-Based Escherichia coli Biosensor.

ABSTRACT: New approaches to deal with drug-resistant pathogenic bacteria are urgent. We studied the antibacterial effect of chitosans against an Escherichia coli quorum sensing biosensor reporter strain and selected a non-toxic chitosan to evaluate its quorum sensing (QS) inhibition activity and its effect on bacterial aggregation. To this end, chitosans of varying degree of acetylation (DA) (12 to 69%) and molecular weight (M_w) (29 to 288 kDa) were studied. Only chitosans of low DA (~12%) inhibited bacterial growth, regardless of their M_w. A chitosan with medium degree of polymerization (named MDP) DA30, with experimental DA 42% and M_w 115 kDa was selected for further QS inhibition and scanning electron microscopy (SEM) imaging studies. M

SUBMITTER: Qin X 

PROVIDER: S-EPMC6164843 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature