Glyphosate Determination by Coupling an Immuno-Magnetic Assay with Electrochemical Sensors.

ABSTRACT: Glyphosate (N-(phosphonomethyl)glycine) is the most frequently used broad-spectrum herbicide worldwide. Its mechanism of action is based on the inhibition of an enzyme that is essential to plant growth. Its intensive use has caused global contamination to occur, which has not only affected the ecosystems, but even food and other objects of common use. Thus, there is a pronounced need for developing analytical methods for glyphosate determination in different matrices. Here, an electrochemical competitive immunoassay, based on the use of antibody-modified magnetic particles, has been developed. Tetramethylbenzidine (TMB) has been used as an enzymatic substrate. The extent of the affinity reaction has been achieved by monitoring the current value, due to the reduction of the enzymatic produc

SUBMITTER: Bettazzi F 

PROVIDER: S-EPMC6164882 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature