Model Senescent Microglia Induce Disease Related Changes in α-Synuclein Expression and Activity.

ABSTRACT: Aging is the most prominent risk factor for most neurodegenerative diseases. However, incorporating aging-related changes into models of neurodegeneration rarely occurs. One of the significant changes that occurs in the brain as we age is the shift in phenotype of the resident microglia population to one less able to respond to deleterious changes in the brain. These microglia are termed dystrophic microglia. In order to better model neurodegenerative diseases, we have developed a method to convert microglia into a senescent phenotype in vitro. Mouse microglia grown in high iron concentrations showed many characteristics of dystrophic microglia including, increased iron storage, increased expression of proteins, such as ferritin and the potassium channel, Kv1.3, increased reactive oxygen s

SUBMITTER: Angelova DM 

PROVIDER: S-EPMC6164966 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature