Ontology highlight
ABSTRACT:
SUBMITTER: Sen Gupta A
PROVIDER: S-EPMC6165600 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Sen Gupta Ayantika A Joshi Gaurav G Pawar Sumit S Sengupta Kundan K
Nucleus (Austin, Tex.) 20180626 1
Eukaryotic cells have 2 to 3 discrete nucleoli required for ribosome synthesis. Nucleoli are phase separated nuclear sub-organelles. Here we examined the role of nuclear Lamins and nucleolar factors in modulating the compartmentalization and dynamics of histone 2B (H2B-ECFP) in the nucleolus. Live imaging and Fluorescence Recovery After Photobleaching (FRAP) of labelled H2B, showed that the depletion of Lamin B1, Fibrillarin (FBL) or Nucleostemin (GNL3), enhances H2B-ECFP mobility in the nucleo ...[more]