Repopulating hematopoietic stem cells from steady-state blood before and after ex vivo culture are enriched in the CD34⁺CD133⁺CXCR4^low fraction.

ABSTRACT: The feasibility of ex vivo expansion allows us to consider the steady-state peripheral blood as an alternative source of hematopoietic stem progenitor cells for transplantation when growth factor-induced cell mobilization is contraindicated or inapplicable. Ex vivo expansion dramatically enhances the in vivo reconstituting cell population from steady-state blood. In order to investigate phenotype and the expression of homing molecules, the expression of CD34, CD133, CD90, CD45RA, CD26 and CD9 was determined on sorted CD34⁺ cells according to CXCR4 ("neg", "low" "bright") and CD133 expression before and after ex vivo expansion. Hematopoietic stem cell activity was determined in vivo on the basis of hematopoietic repopulation of primary and secon

SUBMITTER: Lapostolle V 

PROVIDER: S-EPMC6165804 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature