SNARE-mediated membrane fusion arrests at pore expansion to regulate the volume of an organelle.

ABSTRACT: Constitutive membrane fusion within eukaryotic cells is thought to be controlled at its initial steps, membrane tethering and SNARE complex assembly, and to rapidly proceed from there to full fusion. Although theory predicts that fusion pore expansion faces a major energy barrier and might hence be a rate-limiting and regulated step, corresponding states with non-expanding pores are difficult to assay and have remained elusive. Here, we show that vacuoles in living yeast are connected by a metastable, non-expanding, nanoscopic fusion pore. This is their default state, from which full fusion is regulated. Molecular dynamics simulations suggest that SNAREs and the SM protein-containing HOPS complex stabilize this pore against re-closure. Expansion of the nanoscopic pore to full fusion can th

SUBMITTER: D'Agostino M 

PROVIDER: S-EPMC6166129 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature