Molecular analysis of oral microflora in patients with primary Sjogren's syndrome by using high-throughput sequencing.

ABSTRACT:

Background

The objective of this study was to characterize the oral microflora profile of primary Sjögren's syndrome (pSS) patients, thereby revealing the connection between oral bacterial composition and dental caries, and to identify the "core microbiome" in the oral cavities of pSS patients and systemic healthy individuals by using a high-throughput sequencing technique.

Methods

Twenty-two pSS patients and 23 healthy controls were enrolled in this study. Their clinical data and oral rinse samples were collected. The V3-V4 hypervariable regions of the bacterial 16S rRNA gene of samples were amplified and analyzed by high-throughput sequencing on the Illumina Miseq PE300 platform.

Results

Both two groups were age- and sex-matched. There were significantly higher deca

SUBMITTER: Zhou Z 

PROVIDER: S-EPMC6166617 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature