Cytotoxic and mutagenic properties of O ⁶-alkyl-2'-deoxyguanosine lesions in Escherichia coli cells.

ABSTRACT: Environmental exposure and cellular metabolism can give rise to DNA alkylation, which can occur on the nitrogen and oxygen atoms of nucleobases, as well as on the phosphate backbone. Although O ⁶-alkyl-2'-deoxyguanosine (O ⁶-alkyl-dG) lesions are known to be associated with cancer, not much is known about how the alkyl group structures in these lesions affect their repair and replicative bypass in vivo or how translesion synthesis DNA polymerases influence the latter process. To answer these questions, here we synthesized oligodeoxyribonucleotides harboring seven O ⁶-alkyl-dG lesions, with the alkyl group being Me, Et, nPr, iPr, nBu, iBu, or sBu, and examined the impact of these lesions on DNA r

SUBMITTER: Wang P 

PROVIDER: S-EPMC6166734 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature