Deconvolution of pro- and antiviral genomic responses in Zika virus-infected and bystander macrophages.

ABSTRACT: Genome-wide investigations of host-pathogen interactions are often limited by analyses of mixed populations of infected and uninfected cells, which lower sensitivity and accuracy. To overcome these obstacles and identify key mechanisms by which Zika virus (ZIKV) manipulates host responses, we developed a system that enables simultaneous characterization of genome-wide transcriptional and epigenetic changes in ZIKV-infected and neighboring uninfected primary human macrophages. We demonstrate that transcriptional responses in ZIKV-infected macrophages differed radically from those in uninfected neighbors and that studying the cell population as a whole produces misleading results. Notably, the uninfected population of macrophages exhibits the most rapid and extensive changes in gene expressi

SUBMITTER: Carlin AF 

PROVIDER: S-EPMC6166801 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature