Isolation, Development, and Genomic Analysis of Bacillus megaterium SR7 for Growth and Metabolite Production Under Supercritical Carbon Dioxide.

ABSTRACT: Supercritical carbon dioxide (scCO₂) is an attractive substitute for conventional organic solvents due to its unique transport and thermodynamic properties, its renewability and labile nature, and its high solubility for compounds such as alcohols, ketones, and aldehydes. However, biological systems that use scCO₂ are mainly limited to in vitro processes due to its strong inhibition of cell viability and growth. To solve this problem, we used a bioprospecting approach to isolate a microbial strain with the natural ability to grow while exposed to scCO₂. Enrichment culture and serial passaging of deep subsurface fluids from the McElmo Dome scCO₂ reservoir in aqueous media under scCO₂ headspace enabled the isolation of spore-formi

SUBMITTER: Freedman AJE 

PROVIDER: S-EPMC6167967 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature