Dataset Information

Fluorescence properties of curcumin-loaded nanoparticles for cell tracking.

ABSTRACT:

Background

Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems.

Materials and methods

In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done.

Results

Transplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking.

Conclusion

This approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies.

SUBMITTER: Mogharbel BF

PROVIDER: S-EPMC6168007 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Publications

Fluorescence properties of curcumin-loaded nanoparticles for cell tracking.

Mogharbel Bassam Felipe BF Francisco Julio Cesar JC Irioda Ana Carolina AC Dziedzic Dilcele Silva Moreira DSM Ferreira Priscila Elias PE de Souza Daiany D de Souza Carolina Maria Costa Oliveira CMCO Neto Nelson Bergonse NB Guarita-Souza Luiz Cesar LC Franco Celia Regina Cavichiolo CRC Nakamura Celso Vataru CV Kaplum Vanessa V Mazzarino Letícia L Lemos-Senna Elenara E Borsali Redouane R Soto Paula A PA Setton-Avruj Patricia P Abdelwahid Eltyeb E de Carvalho Katherine Athayde Teixeira KAT

International journal of nanomedicine 20180928

<h4>Background</h4>Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems.<h4>Materials and methods</h4>In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-AD ...[more]

PMID: 30319253

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Project description:Thermally activated delayed fluorescence (TADF) molecules offer nowadays a powerful tool in the development of novel organic light emitting diodes due to their capability of harvesting energy from non-emissive triplet states without using heavy-metal complexes. TADF emitters have very small energy difference between the singlet and triplet excited states, which makes thermally activated reverse intersystem crossing from the triplet states back to the singlet manifold viable. This mechanism generates a long-lived delayed fluorescence component which can be explored in the sensing of oxygen concentration, local temperature, or used in time-gated optical cell-imaging, to suppress interference from autofluorescence and scattering. Despite this strong potential, until recently the application of TADF outside lighting devices has been hindered due to the low biocompatibility, low aqueous solubility and poor performance in polar media shown by the vast majority of TADF emitters. To achieve TADF luminescence in biological media, careful selection or design of emitters is required. Unfortunately, most TADF molecules are not emissive in polar media, thus complexation with biomolecules or the formation of emissive aggregate states is required, in order to retain the delayed fluorescence that is characteristic of these compounds. Herein, we demonstrate a facile method with great generalization potential that maintains the photophysical properties of solvated dyes by combining luminescent molecules with polymeric nanoparticles. Using an established swelling procedure, two known TADF emitters are loaded onto polystyrene nanoparticles to prepare TADF emitting nanomaterials able to be used in live-cell imaging. The obtained particles were characterized by optical spectroscopy and exhibited the desired TADF emission in aqueous media, due to the polymeric matrix shielding the dye from solvent polarity effects. The prepared nanoparticles were incubated with live human cancer cells and showed very low cytotoxicity and good cellular uptake, thus making fluorescence microscopy imaging possible at low dye concentrations.

Dataset Information

Fluorescence properties of curcumin-loaded nanoparticles for cell tracking.

Background

Materials and methods

Results

Conclusion

Publications

Fluorescence properties of curcumin-loaded nanoparticles for cell tracking.

Similar Datasets

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