Generation of controllable gaseous H₂S concentrations using microfluidics.

ABSTRACT: Hydrogen sulfide (H₂S) plays an important role as an intercellular and intracellular signaling molecule, yet its targets are not well understood. As a molecule it easily evaporates and it is hard to acquire stable concentration for in vitro studies, constituting a major problem for the field to identify its downstream targets and function. Here we develop a microfluidic system that can provide consistent and controllable H₂S levels in contrast to the current method of delivering large bolus doses to cells. The system relies on the permeability of H₂S gas through a polydimethylsiloxane thin membrane. A hydrogen sulfide donor, sodium hydrosulfide, is perfused in the microchannels below the gas permeable membrane and gaseous H₂S diffuses across the

SUBMITTER: Christoforidis T 

PROVIDER: S-EPMC6168208 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature