Rapid constriction of the selectivity filter underlies C-type inactivation in the KcsA potassium channel.

ABSTRACT: C-type inactivation is a time-dependent process observed in many K⁺ channels whereby prolonged activation by an external stimulus leads to a reduction in ionic conduction. While C-type inactivation is thought to be a result of a constriction of the selectivity filter, the local dynamics of the process remain elusive. Here, we use molecular dynamics (MD) simulations of the KcsA channel to elucidate the nature of kinetically delayed activation/inactivation gating coupling. Microsecond-scale MD simulations based on the truncated form of the KcsA channel (C-terminal domain deleted) provide a first glimpse of the onset of C-type inactivation. We observe over multiple trajectories that the selectivity filter consistently undergoes a spontaneous and rapid (within 1-2 µs) transition to

SUBMITTER: Li J 

PROVIDER: S-EPMC6168234 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature