Metabolism of a synthetic compared with a natural therapeutic pulmonary surfactant in adult mice.

ABSTRACT: Secreted pulmonary surfactant phosphatidylcholine (PC) has a complex intra-alveolar metabolism that involves uptake and recycling by alveolar type II epithelial cells, catabolism by alveolar macrophages, and loss up the bronchial tree. We compared the in vivo metabolism of animal-derived poractant alfa (Curosurf) and a synthetic surfactant (CHF5633) in adult male C57BL/6 mice. The mice were dosed intranasally with either surfactant (80 mg/kg body weight) containing universally ¹³C-labeled dipalmitoyl PC (DPPC) as a tracer. The loss of [U¹³C]DPPC from bronchoalveolar lavage and lung parenchyma, together with the incorporation of ¹³C-hydrolysis fragments into new PC molecular species, was monitored by electrospray ionization tandem mass spectrometry. The cata

SUBMITTER: Madsen J 

PROVIDER: S-EPMC6168297 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature