5,6-DiHETE attenuates vascular hyperpermeability by inhibiting Ca²⁺ elevation in endothelial cells.

ABSTRACT: Although more than 100 lipid metabolites have been identified, their bioactivities remain unknown. In a previous study, we discovered that the production of several lipid metabolites in the intestines dramatically changed in colitis. Of these metabolites, 5,6-dihydroxyeicosatetraenoic acid (DiHETE) possesses novel anti-inflammatory activity in the vasculature. In this study, we used mouse and human umbilical vein endothelial cell (HUVEC) models to elucidate the mechanisms underlying the vascular activity of lipid metabolites, particularly those related to the release of histamine, a major proinflammatory mediator that stimulates endothelial cells to produce NO, a mediator of vascular relaxation and hyperpermeability, by activating intracellular Ca²⁺ concentration-dependent signa

SUBMITTER: Hamabata T 

PROVIDER: S-EPMC6168302 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature