COMRADES determines in vivo RNA structures and interactions.

ABSTRACT: The structural flexibility of RNA underlies fundamental biological processes, but there are no methods for exploring the multiple conformations adopted by RNAs in vivo. We developed cross-linking of matched RNAs and deep sequencing (COMRADES) for in-depth RNA conformation capture, and a pipeline for the retrieval of RNA structural ensembles. Using COMRADES, we determined the architecture of the Zika virus RNA genome inside cells, and identified multiple site-specific interactions with human noncoding RNAs.

SUBMITTER: Ziv O 

PROVIDER: S-EPMC6168409 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature