Solar simulated light exposure alters metabolization and genotoxicity induced by benzo[a]pyrene in human skin.

ABSTRACT: Skin is a major barrier against external insults and is exposed to combinations of chemical and/or physical toxic agents. Co-exposure to the carcinogenic benzo[a]pyrene (B[a]P) and solar UV radiation is highly relevant in human health, especially in occupational safety. In vitro studies have suggested that UVB enhances B[a]P genotoxicity by activating the AhR pathway and overexpressing the cytochrome P450 enzymes responsible for the conversion of B[a]P into DNA damaging metabolites. Our present work involved more realistic conditions, namely ex vivo human skin explants and simulated sunlight (SSL) as a UV source. We found that topically applied B[a]P strongly induced expression of cutaneous cytochrome P450 genes and formation of DNA adducts. However, gene induction was significantly reduce

SUBMITTER: von Koschembahr A 

PROVIDER: S-EPMC6168490 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature