Drug screening of biopsy-derived spheroids using a self-generated microfluidic concentration gradient.

ABSTRACT: Performing drug screening of tissue derived from cancer patient biopsies using physiologically relevant 3D tumour models presents challenges due to the limited amount of available cell material. Here, we present a microfluidic platform that enables drug screening of cancer cell-enriched multicellular spheroids derived from tumour biopsies, allowing extensive anticancer compound screening prior to treatment. This technology was validated using cell lines and then used to screen primary human prostate cancer cells, grown in 3D as a heterogeneous culture from biopsy-derived tissue. The technology enabled the formation of repeatable drug concentration gradients across an array of spheroids without external fluid actuation, delivering simultaneously a range of drug concentrations to multiple si

SUBMITTER: Mulholland T 

PROVIDER: S-EPMC6168499 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature