Chemogenetic generation of hydrogen peroxide in the heart induces severe cardiac dysfunction.

ABSTRACT: Oxidative stress plays an important role in the pathogenesis of many disease states. In the heart, reactive oxygen species are linked with cardiac ischemia/reperfusion injury, hypertrophy, and heart failure. While this correlation between ROS and cardiac pathology has been observed in multiple models of heart failure, the independent role of hydrogen peroxide (H₂O₂) in vitro and in vivo is unclear, owing to a lack of tools for precise manipulation of intracellular redox state. Here we apply a chemogenetic system based on a yeast D-amino acid oxidase to show that chronic generation of H₂O₂ in the heart induces a dilated cardiomyopathy with significant systolic dysfunction. We anticipate that chemogenetic approaches will enable future studies of in

SUBMITTER: Steinhorn B 

PROVIDER: S-EPMC6168530 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature