Control of proline utilization by the Lrp-like regulator PutR in Caulobacter crescentus.

ABSTRACT: Cellular metabolism recently emerged as a central player modulating the bacterial cell cycle. The Alphaproteobacterium Caulobacter crescentus appears as one of the best models to study these connections, but its metabolism is still poorly characterized. Considering that it lives in oligotrophic environments, its capacity to use amino-acids is often critical for its growth. Here, we characterized the C. crescentus PutA bi-functional enzyme and showed that it is required for the utilization of proline as a carbon source. We also found that putA transcription and proline utilization by PutA are strictly dependent on the Lrp-like PutR activator. The activation of putA by PutR needs proline, which most likely acts as an effector molecule for PutR. Surprisingly, we also observed that an over-pro

SUBMITTER: Mouammine A 

PROVIDER: S-EPMC6168545 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature