Bridging of membrane surfaces by annexin A2.

ABSTRACT: The protein-mediated formation of membrane contacts is a crucial event in many cellular processes ranging from the establishment of organelle contacts to the docking of vesicles to a target membrane. Annexins are Ca²⁺ regulated membrane-binding proteins implicated in providing such membrane contacts; however, the molecular basis of membrane bridging by annexins is not fully understood. We addressed this central question using annexin A2 (AnxA2) that functions in secretory vesicle exocytosis possibly by providing membrane bridges. By quantitatively analyzing membrane contact formation using a novel assay based on quartz crystal microbalance recordings, we show that monomeric AnxA2 can bridge membrane surfaces Ca²⁺ dependently. However, this activity depends on an oxida

SUBMITTER: Grill D 

PROVIDER: S-EPMC6168566 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature