A systems-approach reveals human nestin is an endothelial-enriched, angiogenesis-independent intermediate filament protein.

ABSTRACT: The intermediate filament protein nestin is expressed during embryonic development, but considered largely restricted to areas of regeneration in the adult. Here, we perform a body-wide transcriptome and protein-profiling analysis to reveal that nestin is constitutively, and highly-selectively, expressed in adult human endothelial cells (EC), independent of proliferative status. Correspondingly, we demonstrate that it is not a marker for tumour EC in multiple malignancy types. Imaging of EC from different vascular beds reveals nestin subcellular distribution is shear-modulated. siRNA inhibition of nestin increases EC proliferation, and nestin expression is reduced in atherosclerotic plaque neovessels. eQTL analysis reveals an association between SNPs linked to cardiovascular disease and re

SUBMITTER: Dusart P 

PROVIDER: S-EPMC6168570 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature