Redox manipulation of the manganese metal in human manganese superoxide dismutase for neutron diffraction.

ABSTRACT: Human manganese superoxide dismutase (MnSOD) is one of the most significant enzymes in preventing mitochondrial dysfunction and related diseases by combating reactive oxygen species (ROS) in the mitochondrial matrix. Mitochondria are the source of up to 90% of cellular ROS generation, and MnSOD performs its necessary bioprotective role by converting superoxide into oxygen and hydrogen peroxide. This vital catalytic function is conducted via cyclic redox reactions between the substrate and the active-site manganese using proton-coupled electron transfers. Owing to protons being difficult to detect experimentally, the series of proton transfers that compose the catalytic mechanism of MnSOD are unknown. Here, methods are described to discern the proton-based mechanism using chemical treatment

SUBMITTER: Azadmanesh J 

PROVIDER: S-EPMC6168772 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature