An Anopheles stephensi Promoter-Trap: Augmenting Genome Annotation and Functional Genomics.

ABSTRACT: The piggyBac transposon was modified to generate gene trap constructs, which were then incorporated into the genome of the Asian malaria vector, Anopheles stephensi and remobilized through genetic crosses using a piggyBac transposase expressing line. A total of 620 remobilization events were documented, and 73 were further characterized at the DNA level to identify patterns in insertion site preferences, remobilization frequencies, and remobilization patterns. Overall, the use of the tetameric AmCyan reporter as the fusion peptide displayed a preference for insertion into the 5'-end of transcripts. Notably 183 - 44882 bp upstream of the An. stephensi v1.0 ab initio gene models, which demonstrated that the promoter regions for the genes of An. stephensi are furth

SUBMITTER: Reid W 

PROVIDER: S-EPMC6169391 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature