Study of mitotic chromatin supports a model of bookmarking by histone modifications and reveals nucleosome deposition patterns.

ABSTRACT: Mitosis encompasses key molecular changes including chromatin condensation, nuclear envelope breakdown, and reduced transcription levels. Immediately after mitosis, the interphase chromatin structure is reestablished and transcription resumes. The reestablishment of the interphase chromatin is probably achieved by "bookmarking," i.e., the retention of at least partial information during mitosis. To gain a deeper understanding of the contribution of histone modifications to the mitotic bookmarking process, we merged proteomics, immunofluorescence, and ChIP-seq approaches. We focused on key histone modifications and employed HeLa-S3 cells as a model system. Generally, in spite of the general hypoacetylation observed during mitosis, we observed a global concordance between the genomic organiz

SUBMITTER: Javasky E 

PROVIDER: S-EPMC6169886 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature