Metagenomic analysis with strain-level resolution reveals fine-scale variation in the human pregnancy microbiome.

ABSTRACT: Recent studies suggest that the microbiome has an impact on gestational health and outcome. However, characterization of the pregnancy-associated microbiome has largely relied on 16S rRNA gene amplicon-based surveys. Here, we describe an assembly-driven, metagenomics-based, longitudinal study of the vaginal, gut, and oral microbiomes in 292 samples from 10 subjects sampled every three weeks throughout pregnancy. Nonhuman sequences in the amount of 1.53 Gb were assembled into scaffolds, and functional genes were predicted for gene- and pathway-based analyses. Vaginal assemblies were binned into 97 draft quality genomes. Redundancy analysis (RDA) of microbial community composition at all three body sites revealed gestational age to be a significant source of variation in patterns of gene abu

SUBMITTER: Goltsman DSA 

PROVIDER: S-EPMC6169887 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature