Polycomb repressive complex 1 shapes the nucleosome landscape but not accessibility at target genes.

ABSTRACT: Polycomb group (PcG) proteins are transcriptional repressors that play important roles in regulating gene expression during animal development. In vitro experiments have shown that PcG protein complexes can compact chromatin to limit the activity of chromatin remodeling enzymes and access of the transcriptional machinery to DNA. In fitting with these ideas, gene promoters associated with PcG proteins have been reported to be less accessible than other gene promoters. However, it remains largely untested in vivo whether PcG proteins define chromatin accessibility or other chromatin features. To address this important question, we examine the chromatin accessibility and nucleosome landscape at PcG protein-bound promoters in mouse embryonic stem cells using the assay for transposase accessibl

SUBMITTER: King HW 

PROVIDER: S-EPMC6169895 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature