Cross-talk among writers, readers, and erasers of m⁶A regulates cancer growth and progression.

ABSTRACT: The importance of RNA methylation in biological processes is an emerging focus of investigation. We report that altering m⁶A levels by silencing either N ⁶-adenosine methyltransferase METTL14 (methyltransferase-like 14) or demethylase ALKBH5 (ALKB homolog 5) inhibits cancer growth and invasion. METTL14/ALKBH5 mediate their protumorigenic function by regulating m⁶A levels of key epithelial-mesenchymal transition and angiogenesis-associated transcripts, including transforming growth factor-β signaling pathway genes. Using MeRIP-seq (methylated RNA immunoprecipitation sequencing) analysis and functional studies, we find that these target genes are particularly sensitive to changes in m⁶A modifications, as altered m⁶A status leads t

SUBMITTER: Panneerdoss S 

PROVIDER: S-EPMC6170038 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature