High-dimensional single cell analysis identifies stem-like cytotoxic CD8⁺ T cells infiltrating human tumors.

ABSTRACT: CD8⁺ T cells infiltrating tumors are largely dysfunctional, but whether a subset maintains superior functionality remains ill defined. By high-dimensional single cell analysis of millions of CD8⁺ T cells from 53 individuals with lung cancer, we defined those subsets that are enriched in tumors compared with cancer-free tissues and blood. Besides exhausted and activated cells, we identified CXCR5⁺ TIM-3^- CD8⁺ T cells with a partial exhausted phenotype, while retaining gene networks responsible for stem-like plasticity and cytotoxicity, as revealed by single cell sequencing of the whole transcriptome. Ex vivo, CXCR5⁺ TIM-3^- CD8⁺ T cells displayed enhanced self-renewal and multipotency compared with more d

SUBMITTER: Brummelman J 

PROVIDER: S-EPMC6170179 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature