MYC-containing amplicons in acute myeloid leukemia: genomic structures, evolution, and transcriptional consequences.

ABSTRACT: Double minutes (dmin), homogeneously staining regions, and ring chromosomes are vehicles of gene amplification in cancer. The underlying mechanism leading to their formation as well as their structure and function in acute myeloid leukemia (AML) remain mysterious. We combined a range of high-resolution genomic methods to investigate the architecture and expression pattern of amplicons involving chromosome band 8q24 in 23 cases of AML (AML-amp). This revealed that different MYC-dmin architectures can coexist within the same leukemic cell population, indicating a step-wise evolution rather than a single event origin, such as through chromothripsis. This was supported also by the analysis of the chromothripsis criteria, that poorly matched the model in our samples. Furthermore, we found that

SUBMITTER: L Abbate A 

PROVIDER: S-EPMC6170393 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature