A toolbox for multiplexed super-resolution imaging of the E. coli nucleoid and membrane using novel PAINT labels.

ABSTRACT: Maintenance of the bacterial homeostasis initially emanates from interactions between proteins and the bacterial nucleoid. Investigating their spatial correlation requires high spatial resolution, especially in tiny, highly confined and crowded bacterial cells. Here, we present super-resolution microscopy using a palette of fluorescent labels that bind transiently to either the membrane or the nucleoid of fixed E. coli cells. The presented labels are easily applicable, versatile and allow long-term single-molecule super-resolution imaging independent of photobleaching. The different spectral properties allow for multiplexed imaging in combination with other localisation-based super-resolution imaging techniques. As examples for applications, we demonstrate correlated super-resolution imagi

SUBMITTER: Spahn CK 

PROVIDER: S-EPMC6170473 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature