Origin of Public Memory B Cell Clones in Fish After Antiviral Vaccination.

ABSTRACT: Vaccination induces "public" antibody clonotypes common to all individuals of a species, that may mediate universal protection against pathogens. Only few studies tried to trace back the origin of these public B-cell clones. Here we used Illumina sequencing and computational modeling to unveil the mechanisms shaping the structure of the fish memory antibody response against an attenuated Viral Hemorrhagic Septicemia rhabdovirus. After vaccination, a persistent memory response with a public VH5JH5 IgM component was composed of dominant antibodies shared among all individuals. The rearrangement model showed that these public junctions occurred with high probability indicating that they were already favored before vaccination due to the recombination process, as shown in mammals. In addition,

SUBMITTER: Magadan S 

PROVIDER: S-EPMC6170628 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature