Efficient generation of stable, heritable gene edits in wheat using CRISPR/Cas9.

ABSTRACT:

Background

The use of CRISPR/Cas9 systems could prove to be a valuable tool in crop research, providing the ability to fully knockout gene function in complex genomes or to precisely adjust gene function by knockout of individual alleles.

Results

We compare gene editing in hexaploid wheat (Triticum aestivum) with diploid barley (Hordeum vulgare), using a combination of single genome and tri-genome targeting. High efficiency gene editing, 11-17% for single genome targeted guides and 5% for tri-genome targeted guides, was achieved in wheat using stable Agrobacterium-mediated transformation. Gene editing in wheat was shown to be predominantly heterozygous, edits were inherited in a Mendelian fashion over multiple generations and no off-target effects were observed. Comparison o

SUBMITTER: Howells RM 

PROVIDER: S-EPMC6171145 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature