Notch2 signal is required for the maintenance of canine hemangiosarcoma cancer stem cell-like cells.

ABSTRACT:

Background

Hemangiosarcoma (HSA) is a malignant tumor derived from endothelial cells which usually shows poor prognosis due to its high invasiveness, metastatic rate and severe hemorrhage from tumor ruptures. Since the pathogenesis of HSA is not yet complete, further understanding of its molecular basis is required.

Results

Here, we identified Notch2 signal as a key factor in maintaining canine HSA cancer stem cell (CSC)-like cells. We first cultured HSA cell lines in adherent serum-free condition and confirmed their CSC-like characteristics. Notch signal was upregulated in the CSC-like cells and Notch signal inhibition by a γ-secretase inhibitor significantly repressed their growth. Notch2, a Notch receptor, was highly expressed in the CSC-like cells. Constitutive activatio

SUBMITTER: Aoshima K 

PROVIDER: S-EPMC6171240 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature