High dissolved oxygen tension triggers outer membrane vesicle formation by Neisseria meningitidis.

ABSTRACT:

Background

Outer membrane vesicles (OMVs) are nanoparticles released by Gram-negative bacteria and can be used as vaccines. Often, detergents are used to promote release of OMVs and to remove the toxic lipopolysaccharides. Lipopolysaccharides can be detoxified by genetic modification such that vesicles spontaneously produced by bacteria can be directly used as vaccines. The use of spontaneous OMVs has the advantage that no separate extraction step is required in the purification process. However, the productivity of spontaneous OMVs by bacteria at optimal growth conditions is low. One of many methods for increasing OMV formation is to reduce the linkage of the outer membrane to the peptidoglycan layer by knocking out the rmpM gene. A previous study showed that for Neisseria meningi

SUBMITTER: Gerritzen MJH 

PROVIDER: S-EPMC6171317 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature