Bioinformatics analysis to identify action targets in NCI-N87 gastric cancer cells exposed to quercetin.

ABSTRACT:

Context

Quercetin exerts antiproliferative effects on gastric cancer. However, its mechanisms of action on gastric cancer have not been comprehensively revealed.

Objective

We investigated the mechanisms of action of quercetin against gastric cancer cells.

Materials and methods

Human NCI-N87 gastric cancer cells were treated with 15 μM quercetin or dimethyl sulfoxide (as a control) for 48 h. DNA isolated from cells was sequenced on a HiSeq 2500, and the data were used to identify differentially expressed genes (DEGs) between groups. Then, enrichment analyses were performed for DEGs and a protein-protein interaction (PPI) network was constructed. Finally, the transcription factors (TFs)-DEGs regulatory network was visualized by Cytoscape software.

Results

A total

SUBMITTER: Zeng Y 

PROVIDER: S-EPMC6171422 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature