Critical evaluation of bioinformatics tools for the prediction of protein crystallization propensity.

ABSTRACT: X-ray crystallography is the main tool for structural determination of proteins. Yet, the underlying crystallization process is costly, has a high attrition rate and involves a series of trial-and-error attempts to obtain diffraction-quality crystals. The Structural Genomics Consortium aims to systematically solve representative structures of major protein-fold classes using primarily high-throughput X-ray crystallography. The attrition rate of these efforts can be improved by selection of proteins that are potentially easier to be crystallized. In this context, bioinformatics approaches have been developed to predict crystallization propensities based on protein sequences. These approaches are used to facilitate prioritization of the most promising target proteins, search for alternative

SUBMITTER: Wang H 

PROVIDER: S-EPMC6171492 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature