Multilayered Engineered Tissue Sheets for Vascularized Tissue Regeneration.

ABSTRACT: A major hurdle in engineering thick and laminated tissues such as skin is how to vascularize the tissue. This study introduces a promising strategy for generating multi-layering engineered tissue sheets consisting of fibroblasts and endothelial cells co-seeded on highly micro-fibrous, biodegradable polycaprolactone membrane. Analysis of the conditions for induction of the vessels in vivo showed that addition of endothelial cell sheets into the laminated structure increases the number of incorporated cells and promotes primitive endothelial vessel growth. In vivo analysis of 11-layered constructs showed that seeding a high number of endothelial cells resulted in better cell survival and vascularization 4 weeks after implantation. Within one week after implantation in vivo</

SUBMITTER: Hong S 

PROVIDER: S-EPMC6171602 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature