Three-Dimensional Spheroid Culture Increases Exosome Secretion from Mesenchymal Stem Cells.

ABSTRACT:

Background

Mass production of exosomes is a prerequisite for their commercial utilization. This study investigated whether three-dimensional (3D) spheroid culture of mesenchymal stem cells (MSCs) could improve the production efficiency of exosomes and if so, what was the mechanism involved.

Methods

We adopted two models of 3D spheroid culture using the hanging-drop (3D-HD) and poly(2-hydroxyethyl methacrylate) (poly-HEMA) coating methods (3D-PH). The efficiency of exosome production from MSCs in the 3D spheroids was compared with that of monolayer culture in various conditions. We then investigated the mechanism of the 3D spheroid culture-induced increase in exosome production.

Results

The 3D-HD formed a single larger spheroid, while the 3D-PH formed multiple smaller

SUBMITTER: Kim M 

PROVIDER: S-EPMC6171656 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature