Point-of-care diagnostic assay for the detection of Zika virus using the recombinase polymerase amplification method.

ABSTRACT: The sudden and explosive expansion of Zika virus (ZIKV) from the African continent through Oceania and culminating in the outbreak in South America has highlighted the importance of new rapid point-of-care diagnostic tools for the control and prevention of transmission. ZIKV infection has devastating consequences, such as neurological congenital malformations in infants born to infected mothers and Guillain-Barré syndrome in adults. Additionally, its potential for transmission through vector bites, as well as from person to person through blood transfusions and sexual contact, are important considerations for prompt diagnosis. Recombinase polymerase amplification (RPA), an isothermal method, was developed as an alternative field-applicable assay to PCR. Here we report the development of a

SUBMITTER: Vasileva Wand NI 

PROVIDER: S-EPMC6171711 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature