Non-invasive evaluation of neurovascular coupling in the murine retina by dynamic retinal vessel analysis.

ABSTRACT:

Background

Impairment of neurovascular coupling (NVC) was recently reported in the context of subarachnoid hemorrhage and may correlate with disease severity and outcome. However, previous techniques to evaluate NVC required invasive procedures. Retinal vessels may represent an alternative option for non-invasive assessment of NVC.

Methods

A prototype of an adapted retinal vessel analyzer was used to assess retinal vessel diameter in mice. Dynamic vessel analysis (DVA) included an application of monochromatic flicker light impulses in predefined frequencies for evaluating NVC. All retinae were harvested after DVA and electroretinograms were performed.

Results

A total of 104 retinal scans were conducted in 21 male mice (90 scans). Quantitative arterial recordings were

SUBMITTER: Albanna W 

PROVIDER: S-EPMC6171857 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature