Development of a Quantitative Assay for the Characterization of Human Collectin-11 (CL-11, CL-K1).

ABSTRACT: Collectin-11 (CL-11) is a pattern recognition molecule of the lectin pathway of complement with diverse functions spanning from host defense to embryonic development. CL-11 is found in the circulation in heterocomplexes with the homologous collectin-10 (CL-10). Abnormal CL-11 plasma levels are associated with the presence of disseminated intravascular coagulation, urinary schistosomiasis, and congenital disorders. Although there has been a marked development in the characterization of CL-11 there is still a scarcity of clinical tools for its analysis. Thus, we generated monoclonal antibodies and developed a quantitative ELISA to measure CL-11 in the circulation. The antibodies were screened against recombinant CL-11 and validated by ELISA and immunoprecipitation of serum and plasma. The be

SUBMITTER: Bayarri-Olmos R 

PROVIDER: S-EPMC6172411 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature