Single-cell transcriptomic analyses reveal distinct dorsal/ventral pancreatic programs.

ABSTRACT: The pancreas of vertebrates is separately derived from both the dorsal and ventral endodermal domains. However, the difference between these two programs has been unclear. Here, using a pancreatic determination gene, Pdx1, driven GFP transgenic mouse strain, we identified Pdx1-GFP highly expressing cells (Pdx1^high) and Pdx1-GFP lowly expressing cells (Pdx1^low) in both embryonic dorsal Pdx1-expressing region (DPR) and ventral Pdx1-expressing region (VPR). We analyzed the transcriptomes of single Pdx1^low and Pdx1^high cells from the DPR and VPR. In the VPR, Pdx1^low cells have an intermediate progenitor identity and can generate hepatoblasts, extrahepatobiliary cells, and Pdx1^high pancreatic progenitor cells. In the DPR,

SUBMITTER: Li LC 

PROVIDER: S-EPMC6172462 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature