Phenotypic and Expressional Heterogeneity in the Invasive Glioma Cells.

ABSTRACT:

Background

Tumor cell invasion is a hallmark of glioblastoma (GBM) and a major contributing factor for treatment failure, tumor recurrence, and the poor prognosis of GBM. Despite this, our understanding of the molecular machinery that drives invasion is limited.

Methods

Time-lapse imaging of patient-derived GBM cell invasion in a 3D collagen gel matrix, analysis of both the cellular invasive phenotype and single cell invasion pattern with microarray expression profiling.

Results

GBM invasion was maintained in a simplified 3D-milieue. Invasion was promoted by the presence of the tumorsphere graft. In the absence of this, the directed migration of cells subsided. The strength of the pro-invasive repulsive signaling was specific for a given patient-derived culture. In th

SUBMITTER: Fayzullin A 

PROVIDER: S-EPMC6172486 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature